mouse cd80  (Miltenyi Biotec)

Journal: Journal of Translational Internal Medicine

Article Title: The role of TIM3 + NK and TIM3 - NK cells in the immune pathogenesis of severe aplastic anemia

doi: 10.2478/jtim-2023-0104

Figure Lengend Snippet: The effect of TIM3 + and TIM3 - NK cells on the function of mDCs. (A) Expression of CD80 and CD86 on mDC cells in the control, TIM3 + and TIM3 - NK groups in newly diagnosed SAA patients. (B) The expression of CD80 and CD86 was significantly decreased after incubation with TIM3 - and TIM3 + NK cells in SAA; especially in the TIM3 - NK group, it was significantly lower than that in the TIM3 + NK groups ( P < 0.01).

Article Snippet: The conjugated antibodies used for functional molecules detection were the following: PerCP-Cy5.5 Mouse Anti-Human CD3 (catalog: 560835), FITC Mouse Anti-Human CD16 (catalog: 561308), PE Mouse Anti-Human NKp46 (catalog: 557991), PE Mouse Anti-Human NKp44 (catalog: 558563), PE Mouse Anti-Human NKG2A (catalog: 555889), PE Mouse Anti-Human CD158a (catalog: 556063), PE Mouse Anti-Human CD158b (catalog: 559785), PE Mouse Anti-Human Perforin (catalog: 556437), PE Mouse Anti-Human Granzyme B (catalog: 561142), APC Mouse Anti-Human CD11c (catalog: 559877), PE Mouse Anti-Human CD80 (catalog: 557227), PE Mouse Anti-Human CD86 (catalog: 555658), PE-Cy7 Mouse Anti-Human CD56 (catalog: 557747), Mouse Anti-Human CD86 (catalog: 555658) and FITC Annexin V Apoptosis Detection Kit (catalog: 556570) were purchased from BD Pharmingen (Franklin Lakes, USA).

Techniques: Expressing, Incubation

Journal: Nature medicine

Article Title: HLA-independent T cell receptors for targeting tumors with low antigen density

doi: 10.1038/s41591-021-01621-1

Figure Lengend Snippet: a. Tumour burden (average radiance) of NALM6/12-4-bearing mice treated with 4 × 105 TRAC-HIT or TRAC-CAR T cells (n = 5), analysed through a 35-day period. b. Tumour burden (average radiance) of MM1S-bearing mice treated with 2 × 105 TRAC-HIT or TRAC-CAR T cells (n = 5), analysed through a 35-day period.

Article Snippet: 6 CD80 and 41BBL expression was detected by FACS using mouse anti-human CD80-PE and 41BBL-PE antibodies (BD).

Techniques: Expressing

Journal: Nature medicine

Article Title: HLA-independent T cell receptors for targeting tumors with low antigen density

doi: 10.1038/s41591-021-01621-1

Figure Lengend Snippet: a. NALM6/12-4-bearing mice were treated with 4x105 TRAC-HIT T cells co-expressing the annotated costimulatory ligand. Tumour burden was quantified weekly over a 35-day period, at week 1 or week 3. Each line represents 5 mice. b. NALM6/12-4-bearing mice were treated with 4×105 CAR T cells (n=5 per group; dot = one mouse) and euthanized at days 10 and 17 after infusion; bone marrow TRAC-HIT or TRAC-CAR T cells and NALM6 cells were analysed and counted by FACS. c. Same as in b, except that bone marrow TRAC-HIT or TRAC-CAR CD4 and CD8 T cells counted by FACS. Two-tailed unpaired Student's t-tests were used for statistical analyses. All data are mean ± s.e.m. Tumor burden of individual animals are shown in Supplementary Fig. 2.

Article Snippet: 6 CD80 and 41BBL expression was detected by FACS using mouse anti-human CD80-PE and 41BBL-PE antibodies (BD).

Techniques: Expressing, Two Tailed Test